Computational neuron models, which predict the electrophysiological effects of electrical stimulation within the brain, have proven useful guide how to deliver deep brain stimulation therapy. However, the models make a number of assumptions with regards to axonal structure and axonal biophysics. In this project, the student will leverage microscopy techniques coupled with fast optical recording dyes to develop an approach to visualize axonal activation during extracellular stimulation, and assess how variation in stimulation parameters affects axonal activation.